B8 (Svedbergssalen)

8:45 – 9:15         Registration

 

9:15 – 9:20         Welcome note. Inger Jonasson (Head of Facility, NGI/SciLifeLab)

9:20 – 9:50         Company presentation: PacBio. SMRT technology and its latest advances. Gerrit Kuhn / Nicola Cahill, PacBio.

9:50 – 10:20       Applications of SMRT at a national core facility - challenges and achievements. Olga Vinnere Pettersson

                               (NGI/SciLifeLab).

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10:20 - 10:50     Coffee break at Navet. Company booths open for presentations

 

10:50 – 11:20     Keynote - Single Molecule Sequencing for human genome variation: from repeat expansions to whole

                                chromosome rearrangements. Matthew Hestand, University of Leuven, Belgium

11:20 – 11:40     Application of PacBio long reads to analyse TP53 mutations in leukemia. Thierry Soussi, KI.

11:40 – 12:00     De novo assembly of endosymbiont genomes; is combining PacBio and whole genome amplification a good

                                strategy? Lisa Klasson, UU.

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12:00 – 13:00     Lunch at Navet. Company booths open for presentations.

 

13:00 – 13:30     Company presentation: BioNano Genomics. Illuminating the “Dark Matter”: mapping of the structural variation of the

                                genome, and de novo assembly. Sven Bocklandt, BioNano Genomics.

13:30 – 14:00     Keynote - SMRT based genome assemblies facilitate genomics studies across vertebrates. Carl-Johan Rubin, UU.

14:00 – 14:20     Studying the dark matter of human structural variation by long-read whole genome sequencing of two Swedish

                                individuals. Adam Ameur, NGI/SciLifeLab.

14:20 – 14:40     SMRT-sequencing and optical mapping to detect heterochromatin in the crow genome. Matthias Weissensteiner, UU.

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14:40 – 15:00     Coffee at Navet. Company booths open for presentations.

 

15:00 – 15:30     Company presentation: DNAnexus. Alessandro Riccombeni, DNAnexus.

15:30 – 15:50     Metabarcoding of fungal communities by SMRT sequencing. Björn Lindahl, SLU.

15:50 – 16:10     SMRT sequencing of a locus under strong balancing selection. Tanja Slotte, SU.

16:10 – 16:20     Concluding remarks

 

Navet

16:20 – 16:30     Short break; move to a different location

16:30 – 18:00     One-to-one project meetings (schedule will be announced earlier on Day 1).

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Program for Day 2

November 17

(Trippelrummet at Navet)

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9.00-9.15         Introduction to workshop; PacBio terminology.

9.15-10.45       Session 1. De Novo Sequencing.

10.45-11.00     Coffee break.

11.00-12.00     Session 2. Methylation analysis.

 

12.00-13.00     Lunch.

 

13.00-14.30     Session 3. Amplicon sequencing and sequence capture (including clinical applications).

14.30-14.45     Coffee break.

14.45-15.45     Session 4. Iso-Seq (Whole-length transcript sequencing of cDNA)

15.45-16.00     Summary and concluding remarks.

 

16.00-17.30     Parallel session 1: SMRT analysis portal – introduction and Q&A for new users.

                             Parallel session 2: One-to-one project meetings (schedule will be announced on Day 1).